HEPES Buffer CAS 7365-45-9, HEPES Buffer, CAS 7365-45-9
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HEPES buffer(4 – (2-hydroxyethyl) – 1-piperazine ethanesulfonic acid) is a zwitterionic sulfonic acid buffer; One of good’s 20 buffers.
7365-45-9
C8H18N2O4S
238.3
230-907-9
| CAS | 7365-45-9 |
| Molecular formula | C8H18N2O4S |
| Molecular weight | 238.3 |
| EIENCS | 230-907-9 |
| Form | powder |
| Melting point | 234-238 °C |
| boling point | / |
| Density | 1.07 g/mL at 20 °C |
| Solubility | H2O: 1 M at 20 °C, clear, colorless |
| PKA | 7.5(at 25℃) |
| Color | White |
| Storage temp | 2-8°C |
HEPES buffer(4-hydroxyethylpiperazineethanesulfonic acid) is a non-ionic amphoteric buffer, highly polar, inert to a variety of chemical reagents and enzymes, does not participate in and does not interfere with the process of biochemical reactions, and is resistant to enzymatic chemical reactions. There is no inhibitory effect on the reaction, so it can be specially used for the research of organelles and highly volatile, pH-sensitive proteins and enzymes.
Cell-cell adhesion, short-term cell aggregation culture, buffer for washing tissues and cells.
Patent CN201310477071.6 discloses a method for preparing porous zinc oxide microspheres by using HEPES buffer(4-hydroxyethylpiperazine ethanesulfonic acid) as a template. The method is simple to operate, and the prepared microspheres have the advantages of uniform size and hierarchical pore structure, etc., and can be used for the production of catalysts and gas sensing elements.
Patent CN201080052103.2 uses HEPES buffer(4-hydroxyethylpiperazine ethanesulfonic acid) and its derivatives to treat cancer pain and suppress the side effects after chemotherapy.
Hydroxyethylpiperazineethanesulfonic acid is a common component of buffers in animal and human cell cultures. Studies have confirmed that HEPES buffer(4-hydroxyethylpiperazine ethanesulfonic acid) has minimal cytotoxicity among all known buffers. The FDA (US Food and Drug Administration) has approved piperazine-based zwitterionic molecules as food additives.
HEPES buffer(4 – (2-hydroxyethyl) – 1-piperazine ethanesulfonic acid) is a zwitterionic sulfonic acid buffer; One of good’s 20 buffers. Hepes buffer is widely used in cell culture, mainly because it can maintain physiological pH value better than bicarbonate buffer, which is also commonly used in cell culture, although the concentration of carbon dioxide (produced by aerobic respiration) changes. Lepe Zuniga et al. An unwanted photochemical process in which hepes buffer produces hydrogen peroxide when exposed to ambient light is reported, which is not a problem in bicarbonate based cell culture buffer. Therefore, it is strongly recommended to place the solution containing hepes buffer in the dark as far as possible to prevent oxidation.
HEPES buffer has the following characteristics：
p K a1 (25 °C) = 3
p K a2 (25 °C) = 7.5
Useful pH range = 2.5 to 3.5 or 6.8 to 8.2
The negligible metal ion binding of hepes buffer makes it an ideal buffer for enzymes that may be inhibited by metal chelation.
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